Date of Award

2016

Publisher

North Dakota State University

Document Type

Thesis

Degree Awarded

Master of Science (MS)

Program

Agricultural and Biosystems Engineering

Department

Agricultural and Biosystems Engineering

College

Agriculture, Food Systems and Natural Resources

Faculty Advisor

Pryor, Scott W.

Description

Two experiments were completed to develop methods for extracting xanthophylls from corn industry co-products, post fermentation (PF) corn oil and corn gluten meal (CGM). A solid phase extraction (SPE) method was used to fractionate a xanthophyll-rich portion of PF corn oil by varying conditioning and eluting solvents used with a diol SPE column. Conditioning with dichloromethane yielded highest xanthophyll fractionation, 86.5%. The elution solvent selected did not impact fractionation based on a two-way ANOVA. Supercritical fluid extraction of xanthohpylls from CGM was modeled using a Box-Behnken design, varying temperature, pressure, and co-solvent ratio. The optimum conditions were determined to be 40 °C, 6820 psi, and 15% co-solvent, which would extract 85.4 µg lutein/g CGM, 2.6 times more lutein than an ethanol and chloroform: dichloromethane solvent extraction. Co-solvent was the most influential extraction parameter and increasing it further could yield higher xanthophyll recovery. With further studies, this work has industrial potential.

Rights

NDSU Policy 190.6.2

Rights Link

https://www.ndsu.edu/fileadmin/policy/190.pdf

Handle Identifier

https://hdl.handle.net/10365/28243

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