Date of Award

2022

Publisher

North Dakota State University

Document Type

Thesis

Degree Awarded

Master of Science (MS)

Program

Plant Pathology

Department

Plant Pathology

College

Agriculture, Food Systems and Natural Resources

Faculty Advisor

Pasche, Julie

Description

Common bacterial blight (CBB) (Xanthomonas axonopodis pv. phaseoli (Xap) and X. fuscans pv. fuscans (Xff), halo blight (Pseudomonas savastanoi pv. phaseolicola (Psp)), and bacterial brown spot (P. syringae pv. syringae (Pss)), are yield-limiting diseases of common bean in North Dakota and Minnesota. The objectives of this research were to optimize a multiplex quantitative PCR (qPCR) assay for rapid detection and quantification of four bacterial pathovars in common bean seed, leaf, and pod tissue; determine the aggressiveness of Xap and Xff isolates; and determine the race types of Psp isolates in North Dakota and Minnesota. A fourplex qPCR assay was optimized, and novel primers and probe were designed for pathovar-specific detection of Pss. Significant differences were observed in Xap/Xff aggressiveness across isolates evaluated and in susceptibility of the differential lines (P <.0001). The 60 Psp isolates were identified as Race 6, the predominant race in the region.

Rights

NDSU policy 190.6.2

Rights Link

https://www.ndsu.edu/fileadmin/policy/190.pdf

Handle Identifier

https://hdl.handle.net/10365/33347

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