Date of Award

2015

Publisher

North Dakota State University

Document Type

Thesis

Degree Awarded

Master of Science (MS)

Program

Plant Pathology

Department

Plant Pathology

College

Agriculture, Food Systems and Natural Resources

Faculty Advisor

Pasche, Julie Sherman

Description

Dry bean anthracnose, caused by Colletotrichum lindemuthianum, is a damaging disease that can cause significant reductions in seed quality and yield. The purpose of this study was to determine the races of C. lindemuthianum isolates collected in North Dakota and to develop a real-time qPCR assay to quantify the pathogen in seed and stem tissue. Race 73 continues to be the most common in North Dakota across 33 isolates collected in 2012. The qPCR assay using primer pair ClF1527/ClR1609 was proven useful for quantifying C. lindemuthianum, and was more specific than a previously developed method. Results from greenhouse and field studies indicated that as seed infection increased, emergence decreased and foliar symptoms and pathogen detection increased. Additionally, field studies demonstrated daughter seed infection increased and yield decreased as seed infection severity increased. Using primer pair ClF1527/ClR1609, the pathogen was detected ten days after planting in greenhouse and field studies.

Rights

NDSU policy 190.6.2

Rights Link

https://www.ndsu.edu/fileadmin/policy/190.pdf

Handle Identifier

https://hdl.handle.net/10365/27882

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