Date of Award
2016
Publisher
North Dakota State University
Document Type
Thesis
Degree Awarded
Master of Science (MS)
Program
Horticulture
Department
Plant Sciences
College
Agriculture, Food Systems and Natural Resources
Faculty Advisor
West, Todd P.
Description
Few other woody plants embody the preeminence of temperate woody plants in garden cultivation like the lilacs. In spite of their relationship, the trees lack the diversity of cultivated floral forms observed within the shrub lineages. Typical selection and cross-pollination schemes within the tree lilacs or between trees and shrubs have failed to yield the diversity of colors and fragrances on a tree form. With somatic fusion in Citrus spp. as a guideline for Syringa spp. protoplast isolation and culture, experiments were designed to optimize the conditions through somatic fusion. Protoplast isolation experiments revealed yield increases with increased exposure to cell wall degrading enzymes as well as losses in viability with increased exposure. Electrofusion experiments yielded somatic hybrids, yet further investigation is necessary to optimize the fusion electroporation settings and beyond.
Recommended Citation
Maren, Nathan Allen, "Symmetric Protoplast Fusion in Interserial Syringa (Oleaceae) Hybridization" (2016). Plant Sciences. 291.
https://digitalcommons.ndsu.edu/plant-sci/291
Rights
NDSU policy 190.6.2
Rights Link
https://www.ndsu.edu/fileadmin/policy/190.pdf
Handle Identifier
https://hdl.handle.net/10365/28026