Date of Award
2011
Publisher
North Dakota State University
Document Type
Master's Paper
Degree Awarded
Master of Science (MS)
Program
Biochemistry
Department
Chemistry and Biochemistry
College
Science and Mathematics
Faculty Advisor
Haring, Stuart J.
Description
Whole genome amplification has been used as a powerful tool to increase the amount of DNA template used for microarrays, STR and SNP assays in clinical and forensic settings. Our laboratory observed that multiple displacement whole genome amplification demonstrated a higher reliability for increasing DNA template than PCR primer extension pre-amplification of human genomic DNA. We also demonstrated a truncated reaction time for whole genome amplification was necessary to decrease artifact, while still increasing authentic DNA species. We determined that molecular crowding using polyethylene glycol and non-human DNA also increased sensitivity of our assays. Combining the modified whole genome amplification protocol with macromolecular crowding increased STR signals with as low as one haploid cell DNA equivalent.
Recommended Citation
Palmer, Megan Frances, "The Optimization of Whole Genome Amplification and Molecular Crowding for Use in Forensic Low Copy Number Samples" (2011). Chemistry and Biochemistry. 67.
https://digitalcommons.ndsu.edu/chem-biochem/67
Rights
NDSU Policy 190.6.2
Handle Identifier
https://hdl.handle.net/10365/29637