Date of Award
2016
Publisher
North Dakota State University
Document Type
Thesis
Degree Awarded
Master of Science (MS)
Department
Health, Nutrition and Exercise Sciences
College
Human Sciences and Education
Faculty Advisor
Rhee, Yeong
Description
Diet-induced obesity (DIO) has been shown to increase DNA methyltransferase (DNMT) expression and DNMT binding at obesity-associated genes. Natural compounds have the potential to reverse obesity-associated gene expression via the regulation of DNA methylation. The objective of this study was to determine the effect of the bioactive compounds of flaxseed on DNMT and obesity-associated gene expression. Sixty C57BL/6J male mice were randomly assigned into one of the following diet groups and fed for eight weeks: 45% kcal fat; 45% kcal fat, 10% whole flaxseed; 45% kcal fat, 6% defatted flaxseed; 45% kcal fat, 4% flaxseed oil; and 16% kcal fat. DNMT1, DNMT3a, DNMT3b, leptin, and peroxisome proliferator-activated receptor (PPAR)-α expression levels in muscle and adipose tissues were determined by real-time PCR. The results indicate that bioactive compounds of flaxseed affect selected gene expression. Further research is needed to identify the specific mechanisms regulating leptin or PPAR-α expression during DIO development.
Recommended Citation
Woodard, Alycia Maureen, "The Role of Flaxseed in the Expression of DNA Methyltransferases, Leptin, and Peroxisome Prolifterator-Activated Receptor Alpha in an Obese Animal Model" (2016). Health Nutrition and Exercise Science. 43.
https://digitalcommons.ndsu.edu/health-nutrition-exercise-sci/43
Rights
NDSU Policy 190.6.2
Rights Link
https://www.ndsu.edu/fileadmin/policy/190.pdf
Handle Identifier
https://hdl.handle.net/10365/27924