Date of Award

2015

Publisher

North Dakota State University

Document Type

Thesis

Degree Awarded

Master of Science (MS)

Program

Plant Sciences

Department

Plant Sciences

College

Agriculture, Food Systems and Natural Resources

Faculty Advisor

Marais, Francois

Description

This study was part of a larger pre-breeding effort to develop new parental materials carrying useful genes for disease resistance and adaptation. Firstly, marker-assisted backcrosses were employed to transfer and pyramid combinations of disease resistance genes (Fhb1, Lr34, Lr53, Sr2, Sr26, Sr39, and Sr50) and a reduced height gene (Rht-B1b) into the variety Norstar. Following the third backcross to Norstar, the various backcrossed progenies were inter-mated to derive progenies having combinations of Fhb1 and Rht-B1b plus the targeted leaf and/or stem rust resistance genes. Five NILs (each carrying Fhb1 and Rht-B1b) that differ for the leaf and stem rust resistance genes they possess were recovered. Secondly, a mapping study using the 9K Illumina Infinium iSelect wheat assay was conducted with a F2 mapping population developed by crossing Lr59-25 (0306/2*CSph1b//CSN1AT1B/3/Thatcher) and Superb. Lr59 was mapped 0.5cM distally from the co-segregating SNPs IWA1495, IWA6704, IWA2098 and IWA969 on wheat chromosome arm 6BS.

Rights

NDSU policy 190.6.2

Rights Link

https://www.ndsu.edu/fileadmin/policy/190.pdf

Handle Identifier

https://hdl.handle.net/10365/27821

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